Prof. Dr. Bilge Ayca Kirmizi, akarabork@yahoo.com 1 Introduction Breast cancer is the most frequently diagnosed form of cancer and is the second leading cause of death caused by cancer in women. In order to diagnose breast cancer type, stage, and grade accurately, examination of tissue biopsies and operation specimens is necessary. The biopsy specimens must be fixed embedded in paraffin blocks, mounted on glass slides and stained. Hematoxylin and Eosin (H&E), is a routine stain used in pathology laboratories all over the globe, which gives a good contrast of a tissue section and is commonly used to identify nuclei and cytoplasm [1]. Nevertheless, histopathological examination of the prapared slides involves laborious, time - consuming processes that are limited by specimen quality and pathologist experience.